Laboratory sample preparation for food preservative testing using SPE

SPE Workflow for Detecting Food Preservatives in Processed Foods

Introduction Food preservatives such as benzoates and sorbates are widely used to inhibit microbial growth in processed foods. Reliable detection of these preservatives is essential for regulatory compliance and quality control. Solid-phase extraction (SPE) provides a robust cleanup and preconcentration step before chromatographic analysis. This blog outlines a streamlined SPE workflow for detecting benzoic acid […]

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Clinical laboratory extraction of urine metabolites using WAX SPE cartridges

Using WAX SPE to Detect Organic Acid Metabolites in Urine

Why Profile Organic Acid Metabolites in Urine? Organic acid metabolites are intermediates of amino acid, fatty acid, and carbohydrate metabolism, and their urinary levels serve as critical biomarkers for inherited metabolic disorders, mitochondrial dysfunction, and nutritional status. Accurate profiling using weak anion exchange (WAX) SPE enables sensitive, selective detection of these acidic compounds. Characteristics of

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Laboratory extraction of veterinary drug residues from milk using HLB SPE

Extraction of Veterinary Drug Residues from Milk Using HLB SPE

Extraction of Veterinary Drug Residues from Milk Using HLB SPE 1. Regulatory Importance of Veterinary Drug Monitoring Veterinary drugs are widely used in dairy production to prevent and treat diseases, but their residues in milk pose potential health risks to consumers, including allergic reactions, antimicrobial resistance, and endocrine disruption. Regulatory agencies such as the FDA,

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SPE cartridge method used to isolate alkaloids from herbal medicine extracts

Developing an SPE Cleanup Protocol for Herbal Medicine Alkaloids

Herbal Matrices: A Unique Analytical Challenge Herbal medicines contain complex chemical mixtures, including alkaloids, flavonoids, tannins, pigments, and other secondary metabolites. This complexity poses significant challenges for targeted analysis. The matrix effect in LC-MS can suppress or enhance ionization of target analytes, leading to inaccurate quantification. Developing a robust solid-phase extraction (SPE) cleanup protocol is

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Laboratory SPE workflow used for PFAS environmental testing

Sample Preparation Strategy for PFAS Analysis Using SPE Cartridges

Introduction to PFAS Contamination Issues Per- and polyfluoroalkyl substances (PFAS) are a group of synthetic chemicals widely used in industrial and consumer products due to their unique water- and oil-repellent properties. However, their extreme persistence in the environment and potential adverse health effects—including immune suppression, thyroid disruption, and certain cancers—have made them a top priority

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Laboratory workflow extracting artificial sweeteners from wastewater with HLB SPE

Using HLB SPE to Monitor Artificial Sweeteners in Wastewater

Environmental Occurrence of Artificial Sweeteners Artificial sweeteners, such as sucralose, acesulfame-K, and saccharin, are widely used as sugar substitutes in food, beverages, and pharmaceuticals. Due to their high water solubility, chemical stability, and resistance to biodegradation, these compounds pass largely unchanged through wastewater treatment plants and have been detected in surface waters, groundwater, and even

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SPE cleanup procedure used for disinfectant chemical analysis in laboratory

SPE Method for Extraction of Quaternary Ammonium Compounds from Disinfectant Products

Overview of QAC Disinfectants and Regulatory Monitoring Quaternary ammonium compounds (QACs) are widely used as active ingredients in disinfectants, sanitizers, and preservatives due to their broad-spectrum antimicrobial activity. With the increased use of QAC-based products, regulatory agencies such as the U.S. Environmental Protection Agency (EPA) and the European Chemicals Agency (ECHA) have established guidelines for

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MCX SPE cartridge extraction of antidepressant drugs from plasma samples in analytical laboratory

Optimizing MCX SPE for Quantification of Antidepressants in Human Plasma by LC-MS/MS

Analytical Challenges in Antidepressant Quantification Quantifying antidepressants such as selective serotonin reuptake inhibitors (SSRIs), serotonin-norepinephrine reuptake inhibitors (SNRIs), and tricyclic antidepressants (TCAs) in human plasma presents significant analytical challenges. These compounds are present at low nanogram-per-milliliter concentrations, requiring high sensitivity and selectivity. Plasma is a complex matrix containing proteins, lipids, salts, and other endogenous compounds

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SPE purification of flavonoids from green tea extracts

SPE Isolation of Flavonoids from Green Tea Samples

Flavonoid Compounds in Green Tea (Catechins) Green tea is rich in flavonoids, particularly catechins such as epigallocatechin gallate (EGCG), epigallocatechin (EGC), epicatechin gallate (ECG), and epicatechin (EC). These polyphenolic compounds contribute to the antioxidant, anti-inflammatory, and cardioprotective properties of green tea. Their isolation and purification are critical for nutritional research, quality control, and functional food

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SPE purification of fish tissue extracts by removing lipids

SPE Cleanup of Lipid-Rich Fish Tissue Extracts

Why Lipid-Rich Fish Tissue Presents Unique Analytical Challenges Fish tissue samples, especially those from fatty species like salmon, mackerel, or herring, present significant challenges for trace analysis due to their high lipid content. Lipids—primarily triglycerides, phospholipids, and free fatty acids—can co-extract with target analytes during solvent extraction, leading to severe matrix effects in LC-MS and

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